| Sub-title | |
| Authors | M Cassar C Vidal |
| Abstract | Aim: Chlamydia and gonorrhea infections are two common sexually transmitted diseases that can be asymptomatic and difficult to diagnose especially in women but can eventually lead to serious problems if left untreated. Current testing for these diseases is not so efficient and can often result in false negative results. In this study, a molecular test was developed for detection of Chlamydia trachomatis and Neisseria gonorrhea in urine. Method: Positive controls as well as urine samples spiked with the organisms were used in the analysis. DNA was extracted from 50mls of urine after concentrating the sample. PCR was performed using two sets of specific primers and fluorescently labeled probes using an ABI7300 real time PCR cycler. Results: PCR amplification was detected for all positive controls as well as from urine samples spiked with the corresponding organism. No amplification was observed in non template controls. Conclusion: In this study a very sensitive method for the detection of Chlamydia and gonorrhea in urine samples was developed. This method is useful for early detection of these two common sexually transmitted diseases. |
Published in: | |
| Journal | Malta Medical Journal |
| Volume | 15 Issue 1-2/suppl. 2003 |
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| Key words | DNA, gonorrhoea, PCR, chlamydia |