- To understand the terms surfactant, surface tension, intrapleural space, intrapleural pressure, pneumothorax, and atelectasis.
- To understand the effect of surfactant on surface tension and lung function.
- To understand how negative intrapleural pressure prevents lung collapse.
1
Select Start to initiate the normal breathing pattern and observe the tracing that develops on the oscilloscope.
2
Select Record Data to display your results in the grid. This data represents breathing in the absence of surfactant.
3
Select Surfactant twice to dispense two aliquots of the synthetic lipids and peptides onto the interior lining of the lungs.
4
Select Start to initiate breathing in the presence of surfactant and observe the tracing that develops.
5
Select Record Data to display your results in the grid.
Predict Question 1
6
Select Surfactant twice to dispense two more aliquots of the synthetic lipids and proteins onto the interior lining of the lungs.
7
Select Start to initiate breathing in the presence of additional surfactant and observe the tracing that develops.
8
Select Record Data to display your results in the grid.
9
Select Clear Tracings to clear the tracing on the oscilloscope.
10
Select Flush to clear the lungs of surfactant from the previous run.
Stop & Think Question
Incorrect
The correct answer is:
Surface tension was sequentially decreased.
Stop & Think Question
Incorrect
The correct answer is:
too low.
11
Select Start to initiate breathing and observe the tracing that develops. Notice the negative pressure condition displayed below the oscilloscope when the lungs inflate.
12
Select Record Data to display your results in the grid.
13
Select the valve on the left side of the glass bell jar to open it.
14
Select Start to initiate breathing and observe the tracing that develops.
15
Select Record Data to display your results in the grid.
Stop & Think Question
Incorrect
The correct answer is:
The intrapleural pressure equalized with the atmospheric pressure.
Predict Question 2
16
Select the valve on the left side of the glass bell jar to close it.
17
Select Start to initiate breathing and observe the tracing that develops.
18
Select Record Data to display your results in the grid.
Stop & Think Question
Incorrect
The correct answer is:
No, it did not.
19
Select the Reset button above the glass bell jar to draw the air out of the intrapleural space and return the lung to its normal resting condition.
20
Select Start to initiate breathing and observe the tracing that develops.
21
Select Record Data to display your results in the grid.
Congratulations! You have completed this Experiment.
Select Submit to record your results in the Lab Report and proceed to the Post-lab Quiz.
Current state of the experiment
In total, no aliquots of surfactant was dispensed to the lungs. The valve connecting the left section of the jar with the atmosphere is closed.